Synthesis of Chitosan Collagen Nanoparticles from Iraqi Catfish (Siluriformes) Skin and Evaluation of their Antibacterial Activity

Document Type : New and original researches in the field of Microbiology.

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Institute of genetic engineering and Biotechnology for Higher Studies, University of Baghdad, Iraq

Abstract

Background: Chitosan-collagen nanoparticles have emerged as promising materials for biomedical applications due to their excellent biocompatibility, biodegradability, and antimicrobial properties.  The synthesis of nanocollagen from Iraqi catfish (Siluriformes) skin using chitosan and sodium tripolyphosphate (STPP) as crosslinking agents represents an innovative approach to creating biocompatible nanoparticles. The study utilizes various analytical techniques such as FTIR, UV-Vis, XRD, and SEM to characterize and understand the properties of nanoparticles. Objective: This study aimed to evaluate the efficacy of nano-collagen in managing infections caused by multidrug-resistant bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. Methodology: Bacterial isolates were collected from patients with wound infections at the Al-Zahrawi Hospital in Maysan. Identification was confirmed using selective media (MacConkey agar and mannitol salt agar) and standard microbiological tests, including Gram staining and biochemical assays. The Vitek-2 system was used for precise bacterial identification. Results: Minimum inhibitory concentration (MIC) tests demonstrated that nano-collagen doubled the antibacterial efficacy of collagen extract. The MIC was 47.5 µg/mL (nano-collagen) vs.  95 µg/mL (collagen extract).  against S. aureus the MIC was 95 µg/mL (nano-collagen) vs. 380 µg/mL (collagen extract). Conclusion: These findings suggest that nanocollagen is a promising biomaterial for regenerative medicine and advanced wound care.

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