New Horizons for the Relationship between Genetic Variations and Two Immune Markers in Spontaneous Abortion among Iraqi Women: A Cross-Sectional Study

Document Type : New and original researches in the field of Microbiology.

Authors

1 Department of Microbiology, College of Medicine, Babylon University, 32001, Iraq

2 Department of Microbiology, College of Medicine, University of Babylon, 51001, Iraq

3 Department of Obstetrics and Gynecology, College of Medicine, University of Babylon, 51001, Iraq

Abstract

Background: Spontaneous abortion (SA) is a significant health concern in Iraq. It can be caused by genetic, immunological, and environmental factors. Objective: The aim of this study is to identify the association between CD34+ and HLA-F levels with Toll-like receptors (TLR2, TLR4, TLR7) polymorphisms and their predisposing to SA. Methodology: A survey involving 200 participants was conducted, comprising 100 women diagnosed with SA at the beginning of pregnancy and 100 pregnant women serving as a control group of the same gestational age, all were referred to two Maternity and Children Hospitals in Diyala and Babylon, Iraq. The age range of participants was 20 to 40 years. ELISA was used to quantify the concentrations of immune parameters CD34+ and HLA-F. PCR-RFLP technique was done to evaluate variations in three Toll-like receptor genes. Results: A statistically significant association (p-value ≤0.001) between CD34+ and HLA-F concentrations and a risk factor for SA. The TLR4 (rs1927914) (G/A) genetic variation has revealed a novel association between the genotype GG (2.97±0.7ng/ml) and SA, which have higher levels of HLA-F concentration in peripheral blood of aborted women at a significant value (P-value ≤0.006) compared with other genotypes. Conclusions: The research showed lower CD34+ and HLA-F concentrations in SA women and a novel association between Toll-like receptor 4 genotype GG and elevated HLA-F concentration in Iraqi women experiencing spontaneous abortion and needs further investigations.

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