Cytotoxic activity of partial purified ExoT toxin produced by P. aeruginosa against MCF-7 and PC3 cancer cell lines

Document Type : New and original researches in the field of Microbiology.

Authors

1 Department of Biology, College of Science, University of Anbar

2 Department of Biology, College of Science, University of Baghdad

Abstract

Background: ExotoxinT (ExoT) is one of these exotoxins secreted through the type three secretion system (T3SS) produced by Pseudomonas aeruginosa. Objectives:This study aims to investigate the ability of exotoxin A extracted from Pseudomonas aeruginosa to inhibit cancer cell lines breast cancer (MCF-7) and prostate cancer (PC3). Methodology: seventy one isolates were isolated from 242 clinical samples. The isolates were identified using various microscopic, biochemical, and cultural diagnostic methods.The toxin was then extracted and purified from the productive isolate. ExoT was purified using ammonium sulfate, ion exchange, and gel filtration chromatography (Sephadex G-150). The MTT test was performed to study its inhibitory effects against cancer cell lines in vitro. Results: The results of the MTT assay refer to half-maximal inhibition concentration (IC50) of partially purified ExotoxinT aganist MCF-7,PC3 and HDFn cells was 84.72, 193.1, and 243.9 μg/ml, respectively. The toxic effect was more significant on breast cancer cells than on prostate cancer cells for the values of IC50 compared to normal cells (HDFn). The effect of partially purified ExotoxinT on some criteria or indicators of apoptosis was evaluated by high-content screening that showed a significant effect when exposed to concentrations of 200, and 100 µg/ml. Conclusion: The effect of partially purified exotoxin T was studied by MTT assay and apoptosis indices by high-content assay and showed significant effects

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