Molecular Identification and Phylogeny of Ec-4 Antigen B-Like Gene of Hydatid Disease Caused by Echinococcus granulosus Isolated from Cattle in Basrah South Iraq
Document Type : New and original researches in the field of Microbiology.
Clinical Laboratory Sciences Department, University of Basrah, Basrah, Iraq
10.21608/ejmm.2025.375182.1563
Abstract
Background: Cystic hydatid disease is an important infection that affects a wide range of animal species and humans in many world countries with low-quality control procedures of Echinococcus granulosus in canines, especially dogs. Objective: The current study was conducted to identify and explore the genetic evolution in E. granulosus based on the utilization of Ec-4 antigen B-like (EgAgB) gene as molecular target. Methodology: The study included 67 expected liver samples cysts. These samples were subjected to PCR and partial gene sequencing of the EgAgB gene. Results: The results revealed the presence of these cysts in 16 (23.9%) using morphological identification. The PCR revealed that only 4/16 (25%) of the samples were positive for the gene. The genetic evolution-based phylogenetic tree showed that the current study isolates were nucleotide-similar to isolates from the neighbor country; Turkey. Conclusion: The data of this work showed continued existence of the hydatid disease in cattle in the examined city, still involved in a bovine-dog cycle and so represents a reservoir of the parasite which can apply dangerous health impacts on consumers in the city.
Moker, H., Farhan, H., Mohammed, S., & Qasim, Q. (2025). Molecular Identification and Phylogeny of Ec-4 Antigen B-Like Gene of Hydatid Disease Caused by Echinococcus granulosus Isolated from Cattle in Basrah South Iraq. Egyptian Journal of Medical Microbiology, 34(4), -. doi: 10.21608/ejmm.2025.375182.1563
MLA
Hanady M. Moker; Huda S. Farhan; Suha H. Mohammed; Qutaiba A. Qasim. "Molecular Identification and Phylogeny of Ec-4 Antigen B-Like Gene of Hydatid Disease Caused by Echinococcus granulosus Isolated from Cattle in Basrah South Iraq", Egyptian Journal of Medical Microbiology, 34, 4, 2025, -. doi: 10.21608/ejmm.2025.375182.1563
HARVARD
Moker, H., Farhan, H., Mohammed, S., Qasim, Q. (2025). 'Molecular Identification and Phylogeny of Ec-4 Antigen B-Like Gene of Hydatid Disease Caused by Echinococcus granulosus Isolated from Cattle in Basrah South Iraq', Egyptian Journal of Medical Microbiology, 34(4), pp. -. doi: 10.21608/ejmm.2025.375182.1563
VANCOUVER
Moker, H., Farhan, H., Mohammed, S., Qasim, Q. Molecular Identification and Phylogeny of Ec-4 Antigen B-Like Gene of Hydatid Disease Caused by Echinococcus granulosus Isolated from Cattle in Basrah South Iraq. Egyptian Journal of Medical Microbiology, 2025; 34(4): -. doi: 10.21608/ejmm.2025.375182.1563