Document Type : New and original researches in the field of Microbiology.
Authors
1
Department of Botany, Faculty of Science, Mansoura University, Mansoura 35516, Egypt; Department of Biology, College of Basic Education, University of Misan, Maysan, Iraq
2
Department of Botany, Faculty of Science, Mansoura University, Mansoura 35516, Egypt
3
Department of Agricultural Chemistry, Faculty of Agriculture, Mansoura University, Mansoura City 35516, Egypt
4
Unit of Genetic Engineering and Biotechnology, Faculty of Science, Mansoura University, Mansoura 35516, Egypt; Department of Biology, Faculty of Science, New Mansoura University, New Mansoura City, Egypt
Abstract
Background: Solenostemma argel (Del.) Hayne is a medicinal plant used in the Egyptian folk medicine. It is rich with phytochemicals that could be used in the green synthesis of copper oxide nanoparticles. Objectives: This article aims to greenly synthesize copper nanoparticles (CuO-NPs) using S. argel aqueous extract, to characterize CuO-NPs and to screen their antimicrobial, cytotoxic and antioxidant activity. Methodology: The CuO-NPs were prepared and characterized using various analytical techniques. The antimicrobial, cytotoxic and antioxidant activities were tested using well diffusion, MTT and DPPH assays, respectively. Results and discussion: The results confirmed the successful green synthesis of copper nanoparticles (CuO-NPs) using S. argel extract that was source of reducing phytochemicals like phenolics and flavonoids that were consumed in the synthesis process. The characterization techniques for CuO-NPs confirmed their size, morphology, crystallinity and stability. The antioxidant activity analysis showed IC50 values of 0.011 mg/mL for S. argel and of 0.478 mg/mL for the prepared nanocomposites that were valuable antioxidants. The prepared CuO-NPs expressed broad antimicrobial spectrum against the pathogenic bacterial strains Staphylococcus aureus, Staphylococcus epidermis, Bacillus cereus, Escherichia coli, Salmonella typhimurium and Klebsiella pneumonia and the pathogenic fungus Candida albicans. CuO-NPs showed potent anticancer potential against MDA-MB-231 breast cancer and SKOVE cells using MTT assay. The CuO-NPs have a significant cytotoxic effect (IC50) of 1184 µg/mL against MDA cells and IC50 of 911.1 µg/mL against SKOV cells. Conclusion: The prepared CuO-NPs could be used as a valuable alternative for antibiotics in addition to their potency as antioxidant and anticancer drugs.
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