Green synthesis of Silver Nanoparticles using Cystoseira trinodis: A Sustainable Approach to develop and characterize natural Antimicrobial Drug

Motaweh, Reham R.; Eltanahy, Eladl G.; El-Shehaby, Omar A.; El-Zayat, Mustafa M

doi:10.21608/ejmm.2025.385006.1643

Green synthesis of Silver Nanoparticles using Cystoseira trinodis: A Sustainable Approach to develop and characterize natural Antimicrobial Drug

Document Type : New and original researches in the field of Microbiology.

Authors

¹ Botany Department, Faculty of Science, Mansoura University, Mansoura 35516, Egypt

² Unit of Genetic Engineering and Biotechnology, Faculty of Science, Mansoura University, Mansoura 35516, Egypt; Biology Department, New Mansoura University, New Mansoura, Dakahlia Governorate 35742, Egypt

10.21608/ejmm.2025.385006.1643

Abstract

Background: Cystoseira trinodis extract provide efficient route for green synthesis of silver nanoparticles due to its furious phytochemical content. Objectives: This research aims to biosynthesize silver nanoparticles (AgNPs) utilizing C. trinodis extract and to assess their biological activity. Methodology: The reduction of silver ions by the algal extract commence the biosynthesis of AgNPs and these nanocomposites were screened for their antioxidant and antimicrobial potential. Results: The formation of AgNPs confirmed by maximum absorption peak at 524 nm using UV-Vis spectroscopy, spherical black spots of AgNPs presented using transmission electron microscope, with an average size of 6.12 to 14.92 nm and a zeta potential of -17.2 mV that suggests that AgNPs are relatively stable. The analysis conducted using Fourier-transform infrared spectroscopy validated the participation of functional groups in the capping and stabilization of the nanoparticles. The algal extract exhibited higher total phenolic and flavonoid contents than that of nanocomposite solution due to usage of these groups in the synthesis of nanoparticles. The extract and the formed nanocomposites were screened against the pathogenic microbial strains, Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa, Klebsiella pneumonia, Salmonella typhi, Escherichia coli, and Candida albicans and the synthesized AgNPs expressed strong antimicrobial efficacy in comparison with conventional antibiotics. Furthermore, the phytochemical screening revealed that palmitic acid was the most prevalent fatty acid (28.02%), followed by oleic acid (26.77%) and linoleic acid (22.29%). Conclusion: The results highlight, C. trinoids is promising in a green synthesis of silver nanoparticles that could be used as natural antimicrobial drug.

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