Gene Expression Variations of mirRNA US5-1 in Cytomegalovirus and mirRNA181a in Humans and Their Use as Prognostic Factors for the Risk of Miscarriage in Women in Diwaniyah Governorate, Iraq

Document Type : New and original researches in the field of Microbiology.

Authors

1 Al-Furat Al-Awsat Technical University, Technical Institute, Diwanya, Iraq

2 Department of Biology, Faculty of Sciences, University of Al- Qadisiyah, Iraq

3 Department of Pathology, College of Medicine, University of Al Qadisiya, Diwaniyah, Iraq

Abstract

Background: Human cytomegalovirus (HCMV) can cause serious complications in pregnant women and their fetuses. Objective: the aim of this works is to study the gene expression role of miRNAUS5-1 and miRNA181a in women with HCMV-associated miscarriages. Methodology:  From January 2024 and September 2024, 50 healthy participants and 50 patients with miscarried were enrolled in this study at of the Maternity and Children's Hospital.  The real-time polymerase chain reaction (RT-PCR) test was used to test viral microRNA and human microRNA to detect genetic differences in their expression in women with HCMV-associated miscarriages. Results: Aborted women with CMV levels of miRNA-181a were substantially higher (P<0.05) than those of control participants, while the mean expression of mir-US5-1 gene in women who had miscarriages due to CMV was 5.49 ± 1.20, this gene was not expressed in the healthy control group Conclusion: Our results proved that there was an important immunological role of gene expression in women with HCMV-associated miscarriages.

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