Molecular Screening of Clinical Klebsiella pneumoniae Isolated from Ramadi Hospitals

Document Type : New and original researches in the field of Microbiology.

Authors

1 Department of Medical Laboratories Techniques, College of Health and Medical Techniques University Of Al-Maarif , Al Anbar,31001, Iraq

2 Ministry of Education - Anbar Education Directorate, Iraq

Abstract

Background: Molecular characterization of bacterial strains is essential for understanding the epidemiology of healthcare-associated infections. Strain-level typing enables the differentiation between epidemic strains, which spread between individuals, and endemic or sporadic strains. Aim: This study aimed to identify clinical Klebsiella pneumoniae strains using Multilocus Sequence Typing (MLST) to enhance epidemiological surveillance. Methodology: Multidrug-resistant (MDR) K. pneumoniae isolates were subjected to molecular characterization using MLST. Sequence analysis was performed in accordance to Pasteur Institute's MLST database protocols. Results: Sequencing of seven housekeeping genes for 10 MDR K. pneumoniae isolates revealed that three distinct sequence types (STs): ST16, ST218, and ST283. These STs were recorded for the first time in Iraq according to the Pasteur MLST database. Conclusion: Phylogenetic analysis by MLST scheme of K.pneumoniae strains with ST283 indicated that they are related to strains which discovered in Brazil with same Sequence Type (STs). While the relatedness of ST16 strains close to strains discover in UK . So, the capability of genetic relatedness of different strains of infectious pathogen is very important for the study of epidemiological and surveillance which essential for developing public health control approaches.

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