Biosynthesis of Zinc Oxide Nanoparticles Produced from Penicillium chrysogenum against Acinetobacter baumannii Isolated from Renal Failure Patients

Document Type : New and original researches in the field of Microbiology.

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Department of Microbiology, Faculty of Sciences, University of Kufa, Najaf, Iraq

Abstract

Background: Patients with renal failure suffer from Infection with some gram negative strains, as the development of bacterial resistance to antibiotics is a major problem that limits the use of antibiotics to treat diseases resulting from bacteria. Objective: To detect the effect of ZnO Nanoparticles synthesized from Penicillium chrysogenum On Acinetobacter species isolated from patients with renal failure. Methodology: 102 specimens collected from renal failure patients from (September 2024 to February 2025) for isolates gram negative bacteria, to synthesis nanoparticles 5 mL of the fungal extract was added to the 100 mL of diluted zinc nitrate solution. Results: Among the isolated Gram-negative pathogens, A. baumannii was the most prevalent species 20(19.6%), highlighting its significant role in infections associated with kidney failure and its resistance to multiple antibiotics.The effectiveness of Zinc Oxide Nanoparticles extracted and manufactured from the fungus , P. chrysogenum against A. baumannii, the Ultraviolet and visible spectroscopy (UV-Vis) was to confirm ZnONPs which The UV-vis spectrum revealed the formation of wavelength peak for ZnONPs is at (379 nm) and absorption peaks at (0.80 nm), and also characterization by Atomic Force Microscopy (AFM) , Fourier Transform Infrared Spectroscopy (FT-IR) Analysis and X-Ray Diffraction Analysis (XRD). Conclusion: Zinc Oxide produced by p. chrysogenum has high efficiency in inhibition, decreasing number and destroying the Acinetobacter species by ZnONPs Produced by this fungus at different concentrations (25, 50, and 100) µg /ml . The largest inhibition zone of ZnONPs was (45mm) by a concentration of (100 µg /ml), whereas the lowest inhibition zone  was (11mm) at the concentration (25 µg/ ml).

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