Document Type : New and original researches in the field of Microbiology.
Authors
1
Microbiology Department, College of Medicine, Mustansiriyah University, Baghdad, Iraq
2
Radiology Techniques Department, College of Health and Medical Techniques, Al-Mustaqbal University, 51001, Babylon, Iraq
3
College of Science, University of Sumer, Iraq
4
College of Science, University of Babylon, Iraq
Abstract
Background: Kaposi's sarcoma and HHV-8 pose significant risks to children with acute lymphoblastic leukemia (ALL), with Th17 cells playing a crucial role in ALL pathogenesis. Objective: The purpose of this study was to ascertain whether the percentage of HHV-8 in patients with acute lymphoblastic leukemia (ALL) was correlated with genetic variations of the IL-17 gene. Methodology: This case-control study in Middle Euphrates, Iraq, involved 50 healthy controls and 100 ALL cases, analyzing IL-17 gene polymorphism and HHV-8 detection using sequencing and PCR. Results: Patients with ALL were 12.6 ± 11.66 years old on average. AHC, or apparently healthy control, had an average age of 14.2±12.62 years. Males accounted up to 59% of ALL, with females making up 41%. The results of the viral genome extraction test showed that 52% of the specimens did not contain a viral genome, whereas 48% did. According to the PCR, the rate of human herpes virus-8 infection was 30.7% (16 out of 52), but the negative result was 69.3% (36 out of 52). DNA polymorphism distributions according to A\A; A\G; A\C; G/C and C/C were 23.3%, 26.7%, 33.3%, 16.7%, and 0%, respectively, in patients with ALL, and 35%, 40%, 15%, 10%, and 0%, respectively, in the AHC group, according to the results of the IL-17 rs2275913 polymorphism. Conclusion: The pathophysiology of ALL may involve polymorphisms in the IL-17 gene, particularly when HHV-8 infection is present as a local confounding factor.
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