Copper Nanoparticles by Bacillus amyloliquefaciens strain D 15 as an antimicrobial agent against Antibiotic Resistance

Document Type : New and original researches in the field of Microbiology.

Authors

1 Deptartment of Clinical and Laboratory Sciences, College of Pharmacy, University of Basrah, Basra, Iraq

2 Deptartment of Pharmaceuticals, College of Pharmacy, University of Basrah, Basra, Iraq

3 Deptartment of Pharmacognosy and Medicinal Plants, College of Pharmacy, University of Basrah, Basra, Iraq

Abstract

Background: Using microorganisms to biosynthesize metallic nanoparticles with controlled monodispersity, well-defined sizes, and forms is an exciting and new environmentally friendly technology. Objective: Producing and characterizing CuNPs from soil strains of Bacillus amyloliquefaciens and two techniques were done to evaluate the antibacterial activity.  Methodology: Producing and characterizing CuNPs from soil strains of Bacillus amyloliquefaciens by Transmission electron microscopy (TEM), FT-IR spectroscopy, UV-visible spectroscopy, and energy dispersive spectroscopy (EDX) were among the techniques used to analyze CuNPs. Two techniques were used to evaluate the antibacterial activity. Results: A color shift to green and greenish yellow from light blue is visible, signifying the direct and quick production of CuNPs. The capping activity of Bacillus amyloliquefaciens biomolecules in the synthesis of Cu-NPs caused a peak for surface plasmon resonance to be seen at 386 and 384 nm in the UV-Vis measurement. The TEM analyses further revealed that the particles were globular and varied in size between 31.07 to 98 nm. Cu-NPs were demonstrated to possess antimicrobial action against Staphylococcus aureus from diabetic foot infections, while have no activity against E. coli and Candida albicans from vaginal swabs. Conclusion: Bacillus amyloliquefaciens strain D 15 effectively produced functional Cu-NPs. Cu-NPs demonstrated their antibacterial efficacy against S. aureus, but they had no biological impact on Candida albicans or E. coli.

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