Evaluation of Interleukin 32 Gene (rs4786370) Polymorphism in Patients with Coronary Artery Disease

Document Type : New and original researches in the field of Microbiology.

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Department of Microbiology, Faculty of Medicine, Karbala University, Iraq

Abstract

Background: Coronary Artery Disease (CAD) is a common cardiac illness that impairs blood flow and oxygen supply to the heart muscle. The occurrence and development of coronary artery disorders are intimately linked to IL-32 and its single nucleotide polymorphisms (SNPs). Objective:  This study aimed to investigate the relationship between the genetic variance of IL-32 gene (rs4786370) T/C and the severity of coronary artery disease. Methodology:  Present study includes 50 CAD patients and 50 of healthy individuals as control group. DNA was extracted from whole blood and used in the conventional Polymerase Chain Reaction method to identify the IL-32 gene (rs4786370) polymorphism using a specific allele method. Results: There were statistically significant variations (P = 0.005) in the genotypes of the IL-32 gene (rs4786370) polymorphism between the research groups. In patients, the CC genotype was greater than in the control group. The TC genotype, with non significant result between studied groups. However, it was shown that the prevalence of the TT genotypes was greater in control than in patients. Furthermore, "C" allele were increased in patients than in the control group. In contrast, a significant difference (P=0.001) was present between the "T" allele and the control group. Conclusion: These study findings  indicate that in patients, the CC genotype and C allele were higher than in the control. Therefore, IL-32 gene (rs4786370) polymorphism may be related with increase coronary artery disease risk.

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