Evaluation of the Effect of Aloin Extract from Aloe vera on the Growth of UTI Isolated Escherichia coli with Antibiotics Resistance Genes

Document Type : New and original researches in the field of Microbiology.

Authors

1 Department of Basic Sciences, College of Dentistry, University of Anbar, Ramadi, Iraq

2 Al-Anbar Directorate of Education, Ministry of Education, Iraq

3 College of Science, University of Anbar, Ramadi, Iraq

Abstract

Background: Plant extract therapy against Escherichia coli infections, have been stated. Antibiotic-resistant Escherichia coli have been reported worldwide, with many serious health problems in both human and animal bodies. Therefore, the use of alternative antibacterial treatment is strongly recommended. One of these alternative treatment pathways lies on the use of plant-based products that have the properties of a bactericidal effect. Aloin, in particular, has displayed a wide range of medical uses including antibiotic activities. This product could be extracted from the leaf of Aloe vera. The aim of this study: is to investigate the effect of aloin extract from Aloe vera on the growth of Escherichia coli isolated from patients with urinary tract infections, with a particular focus on comparing its antimicrobial efficacy against commonly used antibiotics. Methodology: This study employed standard microbiological techniques to assess the inhibitory effect of aloin on E. coli strains, including the measurement of minimum inhibitory concentration (MIC) and zone of inhibition in comparison to widely prescribed antibiotics. Additionally, the potential synergistic effects of combining aloin with existing antibiotics were explored to evaluate whether aloin could enhance the antimicrobial activity of traditional drugs, potentially offering a new approach to overcoming bacterial resistance. Results: showed the aloin extract had significant bactericidal activity against E. coli isolates with resistance genes. This result was confirmed using the zone inhibition method and the MIC method. Conclusion: The Synergistic ability of aloin besides antibiotic agents, against bacterial isolates was confirmed using the Fractional Inhibitory Concentration test.

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