Relative miRNA-155-5P Expression and Level of CXCL9, CXCL10 in Patients with Cutaneous leishmaniasis before and after Pentostam Treatment

Document Type : New and original researches in the field of Microbiology.

Authors

1 College of Dentistry, University of Diyala, Diyala, Iraq

2 Department of Biology, College of Science, Tikrit University, Tikrit, Iraq

Abstract

Background: MicroRNAs regulate several immune response pathways in patients with cutaneous leishmaniasis before and after treatment. Objective: The study aimed to estimate the level of immune response before and after treatment with Pentostam by assessing the levels of miRNA-155-5P, CXCL9, CXCL10.  Methodology: This study was conducted on 60 volunteers, 30 with cutaneous leishmaniasis were tested before and after treatment and 30 as a control group. ELISA was performed to determine the level of CXCL9, CXCL10, and the gene expression of miRNA-155-5P was estimated using qRT-PCR. Results: The results of the current study showed a significant increase in relative miRNA-155-5P expression in the control group with patients before treatment (p=0.001) and after treatment(p=0.001). level of CXCL9 was significantly higher in the control group compared to the patient's two groups, p=0.001 and p=0.001, respectively. The level of CXCL10 in the control group increased significantly compared to the patient's groups before treatment (p=0.001) and after treatment (p=0.004). Conclusion: We conclude from the results of the current study that miRNA-155-5P and the immune markers; CXCL9, CXCL10, could constitute a possible therapeutic target or limit the progress of leishmaniasis.

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