Association of CATSPER Gene Polymorphisms with Immunological and Unexplained Infertile Men Before In vitro Sperm Preparation

Abdulghafoor, Hind A.; Aziz, Ismail H.; Al-Dujaily, Saad S.

doi:10.21608/ejmm.2025.408919.1806

Association of CATSPER Gene Polymorphisms with Immunological and Unexplained Infertile Men Before In vitro Sperm Preparation

Document Type : New and original researches in the field of Microbiology.

Authors

¹ Department of Pathology and Forensic Medicine, College of Medicine, University of Fallujah, Baghdad, Iraq

² Genetic Engineering and Biotechnology Institute, Baghdad University, Iraq

³ Department of Physiology, High Institute of Infertility Diagnosis and Art, Al-Nahrain University, Baghdad, Iraq

10.21608/ejmm.2025.408919.1806

Abstract

Background: Approximately fifty percent of infertility cases are male, and the immunological and unexplained kinds are more difficult to diagnose and treat. CATSPER genes play a crucial role in sperm motility and male fertility. Objective: This study focuses on identifying the Single Nucleotide Polymorphism (rs1893316) and (rs2845570) of CATSPER gene in men with immunological and unexplained infertility and assessing their potential association with altered sperm parameters. Methodology: Following semen analysis, 75 men with immunological factors (ASA) and 50 healthy fertile control were enrolled in the present study. The determinations of the SNP (rs1893316) and (rs2845570) of CATSPER were carried out by reverse transcription quantitative polymerase chain reaction (RT-qPCR) of semen samples. Results: The results revealed that the rs1893316 G/A genotypes in both groups exhibit a large deviation from Hardy-Weinberg equilibrium (HWE), There may be a link between the condition and the genotype because the patient group showed a more significant deviation (p = 0.001) than the control group, which showed a modest deviation (p = 0.04). Notable variations were seen in both the patient and control groups, indicating that the SNP rs2845570 is in Hardy-Weinberg equilibrium for both. Patients were more likely to have the heterozygous GT genotype, and they were more likely to have the mutant TT genotype than controls. These variations point to possible connections with the condition under investigation. Conclusion: According to the study, CATSPER polymorphisms are associated with male infertility, namely rs2845570.

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